{"id":8279,"date":"2024-01-23T09:56:06","date_gmt":"2024-01-23T09:56:06","guid":{"rendered":"https:\/\/www-f5.ijs.si\/sted"},"modified":"2024-01-23T10:24:59","modified_gmt":"2024-01-23T10:24:59","slug":"sted","status":"publish","type":"page","link":"https:\/\/www-f5.ijs.si\/en\/sted","title":{"rendered":"Two-photon 3D STED super-resolution microscope"},"content":{"rendered":"\n<p class=\" eplus-wrapper\"><\/p>\n\n\n\n<figure class=\" wp-block-image size-full eplus-wrapper\"><img loading=\"lazy\" decoding=\"async\" width=\"478\" height=\"205\" src=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg\" alt=\"\" class=\"wp-image-6158\" srcset=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg 478w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12-300x129.jpg 300w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12-200x86.jpg 200w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12-400x172.jpg 400w\" sizes=\"auto, (max-width: 478px) 100vw, 478px\" \/><\/figure>\n\n\n\n<p class=\" eplus-wrapper\">In March 2017, a two-photon 3D STED super-resolution microscope was installed at the F5 department, providing fluorescent imaging and nanospectroscopy analysis at spatial resolution of 30 nm. Currently, it is used most frequently by the H2020 SmartNano Tox project to research interactions between nanoparticles and live lung epithelium cells to understand how inhalation of different nanoparticles drives the mechanisms of disease development. With this microscope we were able to observe for the first time the breakdown of cell membranes due to nanoparticles, their coating and movement through the epithelium layer. In the scope of other projects (ARRS, MG, SPS), we use the two-photo excitation and detection with the 100 ps resolution to develop and optimise demonstrators and prototypes of ophthalmic lasers based on new technologies.<\/p>\n\n\n\n<figure class=\" wp-block-image size-large eplus-wrapper\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"666\" src=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1024x666.jpeg\" alt=\"\" class=\"wp-image-3752\" srcset=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1024x666.jpeg 1024w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-300x195.jpeg 300w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-768x500.jpeg 768w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1536x999.jpeg 1536w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-2048x1332.jpeg 2048w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-200x130.jpeg 200w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-400x260.jpeg 400w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-600x390.jpeg 600w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-800x520.jpeg 800w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1000x651.jpeg 1000w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1200x781.jpeg 1200w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1400x911.jpeg 1400w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1600x1041.jpeg 1600w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-1800x1171.jpeg 1800w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/sted-z-bostjanom_fin_c-2000x1301.jpeg 2000w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\" eplus-wrapper\">\ufeff<\/p>\n\n\n\n<p class=\" eplus-wrapper\">\ufeff<\/p>\n\n\n\n<figure class=\" wp-block-image size-large eplus-wrapper\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" src=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1024x683.jpeg\" alt=\"\" class=\"wp-image-3751\" srcset=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1024x683.jpeg 1024w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-300x200.jpeg 300w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-768x512.jpeg 768w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1536x1024.jpeg 1536w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-2048x1365.jpeg 2048w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-200x133.jpeg 200w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-400x267.jpeg 400w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-600x400.jpeg 600w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-800x533.jpeg 800w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1000x667.jpeg 1000w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1200x800.jpeg 1200w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1400x933.jpeg 1400w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1600x1067.jpeg 1600w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-1800x1200.jpeg 1800w, https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/08\/e01_s02_la4_tio2-alexa647_xy_highres_a1_sted-panorama-kompozit-f-2000x1333.jpeg 2000w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>In March 2017, a two-photon 3D STED super-resolution microscope was installed at the F5 department, providing fluorescent imaging and nanospectroscopy analysis at spatial resolution of 30 nm. Currently, it is used most frequently by the H2020 SmartNano Tox project to research interactions between nanoparticles and live lung epithelium cells to understand how inhalation of different [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"_editorskit_title_hidden":false,"_editorskit_reading_time":0,"_editorskit_is_block_options_detached":false,"_editorskit_block_options_position":"{}","editor_plus_copied_stylings":"{}","footnotes":""},"class_list":["post-8279","page","type-page","status-publish","hentry"],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Two-photon 3D STED super-resolution microscope - In\u0161titut Jo\u017eef Stefan<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www-f5.ijs.si\/en\/sted\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Two-photon 3D STED super-resolution microscope - In\u0161titut Jo\u017eef Stefan\" \/>\n<meta property=\"og:description\" content=\"In March 2017, a two-photon 3D STED super-resolution microscope was installed at the F5 department, providing fluorescent imaging and nanospectroscopy analysis at spatial resolution of 30 nm. Currently, it is used most frequently by the H2020 SmartNano Tox project to research interactions between nanoparticles and live lung epithelium cells to understand how inhalation of different [&hellip;]\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www-f5.ijs.si\/en\/sted\" \/>\n<meta property=\"og:site_name\" content=\"In\u0161titut Jo\u017eef Stefan\" \/>\n<meta property=\"article:modified_time\" content=\"2024-01-23T10:24:59+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/www-f5.ijs.si\/wp-content\/uploads\/2023\/12\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted\",\"url\":\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted\",\"name\":\"Two-photon 3D STED super-resolution microscope - In\u0161titut Jo\u017eef Stefan\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/www-f5.ijs.si\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg\",\"datePublished\":\"2024-01-23T09:56:06+00:00\",\"dateModified\":\"2024-01-23T10:24:59+00:00\",\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/en\\\/sted#primaryimage\",\"url\":\"https:\\\/\\\/www-f5.ijs.si\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg\",\"contentUrl\":\"https:\\\/\\\/www-f5.ijs.si\\\/wp-content\\\/uploads\\\/2023\\\/12\\\/vloga-celi-nega-lipidnega-metabolizma-pri-amiotrofi-ni-lateral-12.jpg\"},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/www-f5.ijs.si\\\/#website\",\"url\":\"https:\\\/\\\/www-f5.ijs.si\\\/\",\"name\":\"In\u0161titut Jo\u017eef Stefan\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/www-f5.ijs.si\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"Two-photon 3D STED super-resolution microscope - In\u0161titut Jo\u017eef Stefan","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www-f5.ijs.si\/en\/sted","og_locale":"en_US","og_type":"article","og_title":"Two-photon 3D STED super-resolution microscope - In\u0161titut Jo\u017eef Stefan","og_description":"In March 2017, a two-photon 3D STED super-resolution microscope was installed at the F5 department, providing fluorescent imaging and nanospectroscopy analysis at spatial resolution of 30 nm. 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